What is Semen Analysis physical Examination and microscope Examination

 Semen Analysis

Sperm sample

Senem's examination is done at the following points.

1. Physical Examination
2. Microscope Examination

1. Physical Examination

1. Quantity:- Normally this is under 3 to 4ml .
2. Viscosity:- Fresh collect semen is very thick and should swell on its own within 15 to 30 minutes.
3. Color:- Generally semen's color is blurred white.
4. ph :-. Mostly it is base (ph= 7.2 to 8.0 )

Sperm Microscope Examination

2. Microscopic Examination:

Microscopic examination should be fought as soon as the semen becomes completely liquid within 15 to 20 minutes of semen collection.

1. Sperm count

Requirement:- 

• Neubauer Chamber
• Cover slip 
• Microscope
• WBC pipette
• Semen Diluting Fluid (Sodium Bicarbonate 5gm, Formalin, Phenol 1ml, Distill Water 100ml)

Procedure:- 

1. After mixing the semen properly, take 0.5 marks in the WBC pipette.
2. It is filled with semen Dilution solution up to 10 marks.
3. Waits for 2 minutes and then count the sperm in the microscope.
4. Count the quadrilaterals with four corners in the Neubauer Chamber.

Normal sperm count - 6 to 15 corors/ml

2. Variance Capability

1. Sperm is examined by placing liquid semen on a microscope slide.
2. The cover slip is placed on the slide and it is sealed with Vaseline.
3. Now see the slide in high power objective and study at least two hundred sperm.
4. Generally 80% of the spermatozoa are shown to have high variability and 20% are slow or very good.

3. Sperm Analysis 

1. Make a smear from the semen on the slide and dry it.
2. Now to remove the music, put 1% chloramine on it for a few minutes.
3. It is dried by pressing it with filter paper.
4. Now stain it for 2 to 5 minutes with the following points.

• Jil Nilson Kabrol Phokison - 2 part
• Eosin Solution in Saturated Alcohol - 1 part.
• Alcohol 95% - 1 part

      5. After washing it with water, stain it                  with taufler's methylene blue for a few            seconds

      6. Wash it dry and then look in the                        microscope.

       7. The head of the sperm is purple and                 the middle part and the tail part                       are of black color.

        8. Now count sperm in each                                    microscopic area, also count                              abnormal and undeveloped sperm                    in each area.