Antibody and Antigen Reaction
Introduction
Antigen cobine with its specific antibody is an observable manner and the reaction between antigen and antibody is specific
Uses : In the body : it from the basis of immunity agents infection disease.
It may lead to tissue injury in some hyper sensitive reaction and auto immune disease
2 Detection and quantitative of either antigen and antibody .
Characteristics :Reaction is specific and Antigen combine only with its homologous antibody . Intier molecular of antigen and antibody react and not the fregments.
Antigen and antibody can combine in verifiny proportion antibody are generally
bia valent all though IgM may have five or more combing sites.
A single antigen can cause different type of antigen, antibody reaction and single antigen can estimulate production of different classes antigen can estimulate production of different classes of immunoglobulin.
Types of antigen and antibody reaction
- Precipitation reaction
- Agglutination Reaction
- Complament fixation test
- Immunofluorescence
- Neutralization
- Radio immuno assay (RIA)
- Enzyme liked immuno sorvent assay (ELISA)
- Immune Chromatography
Types of agglutination test
- Slide agglutination test
- Tube agglutination test
- Heterphilic agglutination test
Passive agglutination test is 3 types
- Letex Agglutination test
- Haem agglutination test
- Coagulation
• Poly stave latex particles are widely employed to adsorb several types of antigens
• Latex particles can also be coated with antibody for detection of antigen
• These tests are used for detection of Hepatitis B antigen , ASO, CRP, RA FACTOR, HCG, bacterial typing ( N. menigitidis) and many other antigen.