Histopathology in Practice BMLT

  1. Sample Reception, 
  2. Sample records
  3. Labelling of histology

Histology

  • The science concerned with the microscopic structure of tissues and organs in relation to their function.

Also called microanatomy


Specimen Reception

Specimen Reception



Specimen Unpacking

Specimen Unpacking

Specimen QC

Specimen QC

Specimen Labelling


Specimen Labelling

Sample Documenting

Sample Documenting

Cassetting

Cassetting

Specimen Dissection the stage 

Specimen Dissection the stage

Dissecting props

  • Cutting board
  • Forceps
  • Ruler
  • Scalpel / knives / saw
  • Inks / dyes
  • Cassettes / lids
  • Biopsy pads / tissue
  • Filter bags
  • Weighing scales

Cut-up dictation

  • Morphology” computer system
  • “Dragon” dictation system
  • Hands-free
  • Just scan the barcode and away we go!!

Specimen Categories

A. Specimens only requiring transfer from container to tissue cassette.
B. Specimens requiring transfer but with standard sampling, counting, weighing or slicing.
C. Simple dissection required with sampling needing a low level of diagnostic assessment and/or preparation.
D. Dissection and sampling required needing a moderate level of assessment.
E. Specimens requiring complex dissection and  sampling methods

Category A Specimens

Category B Specimens

Catagory C Specimens 

Category D Specimens

Category E Specimens


Specimen Sampling

  • Literally, taking a sample of the tissue
  • Representative

  • Generally, fewer blocks required if the tissue looks uniform throughout (for benign cases)
  • Sampling “rules”

Specimen Storage



  • Ventilated storage units
  • Largest buckets lower shelves
  • Units are in date (week) order
  • 5-weeks’ worth of storage
  • Only authorised specimens are discarded after 5 weeks
  • Any outstanding cases are stored separately until further notice

Processing

Processing

Processing programs

Processing programs

Embedding

Embedding

Final Block preparation

Final Block preparation